Lyles & King was founded in 2015 in New York’s Lower East Side by Isaac Lyles. The international and intergenerational gallery program focuses on presenting emerging artists alongside under-recognized artists such as Mira Schor and Rosa Loy. The gallery is predicated on mounting ambitious, often international, exhibitions that frequently address issues of gender, identity, and bodily experience. In September 2020, the gallery opened a new space at 21 Catherine Street. The new gallery features a larger main exhibition space, viewing rooms, and outdoor sculpture and performance space. In September 2021, the gallery expanded to a second exhibition space at 19 Henry Street.

Gallery artists are in the collections of the Centre Pompidou, Paris; Solomon R. Guggenheim Museum, New York; Museum of Modern Art, New York; Museum of Modern Art, Warsaw; San Francisco Museum of Modern Art, San Francisco; Hammer Museum, Los Angeles; Whitney Museum of American Art, New York; and many others. Gallery exhibitions are often featured in local and international press.